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Landscape of Interacting Microbes - Protocols

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Lab protocols

Making media and buffers

• SB buffer for gel electrophoresis

DNA/RNA extraction

• Extraction of high molecular weight DNA from bacterial cultures

PCR

• Colony PCR/16S Full-length 27F/1492R
• 16S V4 515F/806R
• 18S V4 TAReuk454FWD1/TAReuk454REV3.1
• ITS 3F/4R

Bioinformatics tutorials

• QIIME2 tutorial